Beilstein J. Org. Chem.2022,18, 1289–1310, doi:10.3762/bjoc.18.135
celastrol, a potent anti-obesity metabolite [42][43]. In two independent studies, transcriptome mining and functional studies in Nicotianabenthamiana were used to identify the CYPs CYP712K1, CYP712K2, CYP712K3, and CYP712K4 capable of oxidising friedelin (8) into polpunonic acid via an aldehyde
increasing availability of high-quality transcriptome and genome data even of non-model plants together with reliable and efficient expression systems in yeast and in Nicotianabenthamiana will facilitate future approaches to fully harness the diversity of triterpenoids and steroids found in plants. In
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Graphical Abstract
Figure 1:
Enzyme function of cytochrome P450 monooxygenases (CYPs). A) Typical net reaction of CYPs, resultin...
Beilstein J. Org. Chem.2017,13, 1770–1780, doi:10.3762/bjoc.13.171
reaction were addressed by isotopic labelling experiments. Heterologous expression of the diterpene synthase in Nicotianabenthamiana resulted in the production of 18-hydroxydolabella-3,7-diene also in planta, while the results from the heterologous expression in E. coli were shown to be reproducible
, revealing that the expression of one and the same terpene synthase in different heterologous hosts may yield different terpene products.
Keywords: biosynthesis; Chitinophaga pinensis; Nicotianabenthamiana; structure elucidation; terpenes; Introduction
Terpene synthases convert a handful of simple linear
this enzyme in in vitro experiments and the first heterologous expression of a bacterial terpene synthase gene in a plant, Nicotianabenthamiana.
Results and Discussion
Characterisation of a diterpene synthase from Chitinophaga pinensis in vitro
The terpene synthase from C. pinensis was heterologously
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Graphical Abstract
Scheme 1:
Germacrene A (1) and its Cope rearrangement to β-elemene (2).